Product
소개
TCEP Reducing Agent Biochemicals
TCEP is the next generation reducing agent. It is used as a hydrochloride salt (TCEP-HCl) with a
molecular weight of 286.65 g/mol.
It is soluble in water, odourless and non-toxic. It is used as a reducing agent to cleave disulphide
bridges during protein chromatography and in SDS-polyacrylamide gel electrophoresis.
TCEP is also used in the tissue homogenization process for RNA isolation.
TCEP has the following benefits over DTT and 2-ME:
• Odourless
• More powerful reducing agent
• Irreversible reducing agent
• More hydrophilic
• More resistant to oxidation.
• It does not reduce metals used in IMAC
• It causes fewer ITC baseline artefacts
• TCEP is particularly useful when labeling cysteine residues with maleimides. TCEP can keep the cysteines from
forming di-sulfide bonds and unlike dithiothreitol and β-mercaptoethanol, it will not react as readily and
in certain conditions with the maleimide.
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