TCEP Reducing Agent        Biochemicals 

TCEP is the next generation reducing agent. It is used as a hydrochloride salt (TCEP-HCl) with a 

molecular weight of 286.65 g/mol.

It is soluble in water, odourless and non-toxic. It is used as a reducing agent to cleave disulphide

bridges during protein chromatography and in SDS-polyacrylamide gel electrophoresis. 

TCEP is also used in the tissue homogenization process for RNA isolation. 

TCEP has the following benefits over DTT and 2-ME: 

•   Odourless 

•   More powerful reducing agent 

•   Irreversible reducing agent 

•   More hydrophilic 

•   More resistant to oxidation.   

•   It does not reduce metals used in IMAC 

•   It causes fewer ITC baseline artefacts 

•   TCEP is particularly useful when labeling cysteine residues with maleimides. TCEP can keep the cysteines from 

    forming di-sulfide bonds and unlike dithiothreitol and β-mercaptoethanol, it will not react as readily and 

    in certain conditions with the maleimide. 

   TCEP Datasheet

   TCEP MSDS