Product
제품
- ViscoStar®
- 제품명: ViscoStar®
- 용도: Online Differential Viscometer
- 메이커: Wyatt Technology
- 카달로그:
소개
ViscoStar®
The next generation online differential viscometer
A highly sensitive, on-line differential viscometer used in conjunction with SEC-MALS-IV to determine the size and conformation of all types of biopolymers, synthetic polymers and even proteins and peptides. The ViscoStar® incorporates multiple novel technologies to provide the highest sensitivity, stability and solvent compatibility of any available viscometer for GPC. Its ease-of-use and serviceability make it the perfect companion for Wyatt's DAWN® light scattering and Optilab® refractive index detectors.
Download "ViscoStar: Innovations in Online Viscometry for GPC".
Another path to polymer and protein characterization: SEC-MALS-IV
When coupled with size-exclusion chromatography and a concentration detector such as the Optilab, theViscoStar derives the intrinsic viscosity (IV) of
polymers. An inherently valuable physico-chemical property in itself, IV is best utilized together with molar mass information from multi-angle light
scattering (SEC-MALS-IV) to derive the hydrodynamic radius, conformation, and branching ratio of a macromolecule in solution.
Key benefits of the ViscoStar
• High sensitivity, equivalent to 100 ng of 100 kDa polystyrene in THF at 1.0 mL/mininjected onto a standard GPC column
• High dynamic range of over 135,000:1. The full measurement range corresponds to over 13 mg/mLof 100 kDa polystyrene in THF
• Extended solvent compatibility with all-316 stainless steel transducers
• Temperature range from 4°C to 70°C for polymers or proteins that require a range oftemperatures
• Minimal band broadening thanks to fast pressure transducers
• Pump pulse suppression to below 4 Pa (hardware alone) or 1 Pa (hardware + software) withoutloss of chromatographic resolution
• Automated capillary bridge tuning with no moving parts, to guarantee a perfectly balancedbridge, every run
• Enhanced thermal stability with drift of <2.5 Pa/hour
SEC-IV without MALS?
Yes, as much as we love MALS for optimal polymer analysis, it's true that there are occasions when it just won't fit the bill.
Universal calibration is a parallel technique to SEC-MALS for determining molecular weight, commonly used to characterize linear polymers.
While universal calibration overcomes some of the problematic issues associated with column calibration by reference molecules, it does not
account for non-ideal column behaviorcaused by chemical solute-column interactions or hyperbranching.
Universal calibration is particularly-well suited to replace MALS for:
• Strongly fluorescent polymers, in particular those not adequately addressed by aDAWN withlong-wavelength (785 nm) laser or a
standard DAWN (685 nm) with fluorescence-blocking filters.
• Weakly-scattering polymer/solvent systems with very low dn/dc values
The Mark-Houwink-Sakurada relationship makes use of empirical relationshipsbetween intrinsic viscosity and molar mass, specific to each polymer
and solvent, to estimate molar massfrom SEC-IV measurements. Of course, the best way to determine MHS coefficients is to measure them using
SEC-MALS-IV and ASTRA!
사양
* Entrained volume of the delay columns. Approximately twice this amount must flow through the instrument until the retained sample elutes. The 16.2 mL option must be requested at time of purchase.
액세서리
Orbit - The Orbit helps conserve mobile phase by programmatically directing the solvent to either a waste bottle or back to the solvent reservoir.
Solaris - Prevents growth of bacteria, fungi or algae in solvents with high efficiency and protects your system from down-time due to contamination.
WISH Injection Module - The Wyatt Injection System High-Pressure (WISH) is ideal for use when an autosampler is not available. The WISH triggers data acquisition by ASTRA upon injection.
관련자료
Viscometry-only
The ViscoStar may be used with any* GPC or HPLC-SEC system as long as the sample does not need to be heatedabove 70°C in order to flow through 0.010" (0.25 mm) i.d. tubing. Combine with an Optilab for theconcentration measurements needed in viscometry calculations.ASTRA supports Universal Calibration as well as molar mass determination using Mark-Houwink-Sakurada coefficients.
SEC-MALS-IV
Combine the ViscoStar with a DAWNor miniDAWN MALS detector for an even more versatile combination that can determine Mark-Houwink-Sakuradaparameters and other measures of conformation and stability. ASTRA handles all the calculations you need for full characterization of your polymers.