Product
소개
microOptilab®
On-line Differential Refractometers for HPLC and UHPLC
Universal Detection for UHPLC and SEC-MALS
Independent of chromaphores or fluorophores, differential Refractive Index (dRI) detectors measure concentrations of all types of samples
in almost all solvents. The microOptilab™ brings the universal detection capabilities, unsurpassed concentration range, and superb sensitivity
and linearity – familiar from the Optilab – to the domain of UHPLC.
Download our Application Note on the microOptilab
SEC-MALS with UHPLC
Like standard SEC, UHPLC-SEC analysis relying on column calibration with molecular standards is unreliable when the standards do not accurately
reflect your samples in terms of conformation, non-ideal (e.g., hydrophobic) column interactions or reversible association. SEC-MALS employs a
microDAWN MALS detector downstream of the SEC column and UV detector, to determine molar mass / molecular weight distributions of
macromolecules independently of column calibration.
Refractive index detection is an important component of any SEC-MALS system, and necessary for analyzing conjugated proteins or polymers devoid of
chromaphores. The microOptilab connects with the microDAWN MALS detector for a complete UHP-SEC-MALS setup that transfers the many benefits
of SEC-MALS analysis to the realm of UHPLC.
Key benefits
• Very low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks
• Extraordinary dRI measurement range, -4.7 x 10-3 to +4.7 x 10-3 RIU (comparable to 25 mg/mL protein in PBS)
• Highly sensitive, ± 1.5 x 10-9 RIU across the entire measurement range
• Measures solvent refractive index, require for MALS calculations
• Measures at the same wavelength as the MALS detector, for maximum accuracy in MALS calculations
• Temperature range 4°C to 65°C for proteins and polymers that require a range of temperatures
• High-resolution, touch-screen front panel displays data graphs and instrument diagnostics, even with the host computer turned off
Unparalleled features
• Considering the extremely small peak volumes of UHPLC, band broadening in the microOptilab is kept to the absolute minimum, at less than 4 µL,
as compared to 20 - 40 µL or more for standard RI detectors. As a result, the superior resolution from UHPLC columns and instruments will not be
compromised with the microOptilab RI detector.
• Even though the microOptilab offers a dRI measurement range orders of magnitude beyond that of other UHPLC dRI detectors, unlike other detectors
there are no range or gain settings in the microOptilab. The full range of instrument detection is always present, and the full sensitivity exists over the
entire range. The small flow cell and proprietary temperature regulation enable stable RI baselines and signals which further enhance the
detector's sensitivity.
• Unlike all other dRI detectors, the microOptilab also measures absolute refractive index in order to determine solvent RI, necessary in light scattering
calculations and useful in assessing the accuracy of mixing of solvent gradients.
• The microOptilab can be operated below ambient temperatures as easily as above ambient. No hassle temperature control can be programmed down to
4°C or as high as 65°C.
• The microOptilab is compatible with all UHPLC systems.
사양
* Short term peak-to-peak noise is measured as per ASTM-E1303-95(2000) using a 0.5 sec collection interval (1-sec time constant) and temperature set to 25°C.
** Peak broadening measured over full width at half max, flow rate = 0.3 mL/min.
액세서리
RI Calibration Kit - Pre-made RI calibration standards are available to speed up RI calibration. Can be used to calibrate any Optilab or third-party
differential RI instrument.
WISH Injection Module - The Wyatt Injection System High-Pressure (WISH) is ideal for use with an Optilab RI detector for the
determination of dn/dc in "batch" mode.
Microbatch Kit - Used to inject samples into the flow cell of a MALS detector, and in determining dn/dc.
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