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Optilab®
제품명: Optilab®
용도: Refractometer for Chromatography and FFF
메이커: Wyatt Technology
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소개

Optilab® 


On-line Differential Refractometers for Chromatography and FFF Separations 


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Universal Detection for Chromatography and FFF Separations 


Independent of chromaphores or fluorophores, differential Refractive Index (dRI) detectors serve as accurate and versatile concentration detectors in all types of solvents. dRI is part of standard instrumentation for a variety of macromolecular characterization techniques including standard chromatography, SEC-MALS, FFF-MALS and CG-MALS.The Optilab® (refractometer with extended range) is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range of every other RI detector for chromatography in existence today, making it a valuable addition to any MALS setup. Using a combination of cutting-edge semiconductor photodiode technology and proprietary computer algorithms, the Optilab achieves an unprecedented combination of sensitivity and range. These features mean that it addresses both standard chromatographic applications and some challenges unique to light scattering measurements such as high concentrations, determination of sample refractive increments (dn/dc) and solvent refractive index.

 

Key Benefits 

•  Extraordinary dRI measurement range, -0.0047 to +0.0047 RIU (comparable to 25 mg/mL protein in PBS)

•  Highly sensitive, ±7.5 x 10-10 RIU across the entire measurement range

•  Measures solvent absolute refractive index, required for MALS calculations

•  Measures at the same wavelength as the MALS detector, for maximum accuracy in MALS calculations

•  Band broadening < 20 µL preserves SEC resolutionTemperature range 4°C to 65°C for proteins and polymers that require a range of temperatures

•  High-resolution, touch-screen front panel displays data graphs and diagnostics, even with the host computer turned off  

 

Unparalleled Features 

•  Even though the Optilab offers a dRI measurement range orders of magnitude beyond that of other dRI detectors, unlike other detectors there are no range or gain settings in the Optilab. The full range of instrument detection is always present, and the full sensitivity exists over the entire range. 

•  The Optilab can also do something no other on-line refractive index detector can: it can measure the absolute refractive index of a solution. In addition, it can measure the dn/dc of a solvent at the same wavelength of light as the light scattering instrument. And just to make sure it can't be matched, you can replace the light source in the Optilab for other wavelengths (e.g., 690, 633, 488, etc.) in just minutes! 

•  The Optilab can go below ambient temperatures as easily as above ambient. No hassle temperature control can be programmed from 4°C to 65°C. 

•  Compatible with all HPLC systems.

 

Revolutionary dRI detection optics 

The Optilab incorporates the break through optical design of the award-winning Optilab dRI detector which makes use of a 512-element diode array and proprietary algorithms, instead of the usual bi-cell design, bringing dRI detection into the 21st century. Later Optilab models added improvements in optical stability, temperature control, LED light source intensity and increased on-board computing power in order to enhance sensitivity and extend the temperature range over the Optilab.  


Minimal loss of resolution 

Each detector downstream of SEC or FFF adds band broadening and dispersion, according to its internal fluidic volume. With a total flow cell volume of only 7.4 µL – 25% less than the leading HPLC refractometers – the Optilab does not impact SEC resolution adversely. This translates into minimized band broadening and better temperature stability. 

사양

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* Short term peak-to-peak noise is measured as per ASTM-E1303-95(2000) using a 0.5 s collection interval (1-s time constant) and temperature set to 25°C.

** Peak broadening measured over full width at half max, flow rate = 0.3 mL/min.


액세서리

RI Calibration Kit - Pre-made RI calibration standards are available to speed up RI calibration. Can be used to calibrate any Optilab or third-party 

                       differential RI instrument.


WISH Injection Module - The Wyatt Injection System High-Pressure (WISH) is ideal for use with an Optilab dRI detector for the determination 

                               of dn/dc in "batch" mode.


Microbatch Kit - Used to inject samples into the flow cell of a MALS detector, and in determining dn/dc.

관련자료

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