Eclipse™  

The ultimate separation system for nanoparticles and macromolecules

 

 

 

 

Product OverView

 

Uniquely Versatile

 

Field-flow fractionation is a uniquely versatile means of size-based separations, capable of fractionating viral particles, liposomes and lipid nanoparticles, emulsions, proteins and aggregates, polysaccharides and technical polymers, engineered nanoparticles, colloidal soil suspensions, chemical mechanical polishing slurries, and many more - with no stationary phase and almost no shear. Separation of biotherapeutics can usually be done in full formulation buffer.

 

The Eclipse™ system is the foundation of FFF-MALS, a powerful technique for separation and comprehensive characterization of macromolecules and nanoparticles from 1 to 1000 nm in size. Eclipse brings FFF to a completely new level, combining exquisite performance and ease of use. Advanced features include the Dilution Control Module™ (DCM) and the ability to switch seamlessly between FFF and size-exclusion chromatography (SEC) separations. The Mobility™ add-on system expands Eclipse’ capabilities to include measurements of charge and zeta potential.

 

 

Product Description

 

Simplicity and quality, with built-in intelligence

 

The Eclipse FFF system combines several modules that are integrated

seamlessly in VISION software:

 

  •  The Eclipse FFF flow instrument

  •  Agilent 1260 Infinity II pump and autosampler

  •  Eclipse FFF channel

 

These modules may be added:

  •  Agilent 1260 fraction collector

  •  Mobility EAF4 system 

 

 

Eclipse instrument

The Eclipse FFF instrument is the heart of the FFF system. It incorporates four highly precise and stable flow controllers that ensure robust and repeatable fractionation runs, without the associated pulsation and inherent complexity and low reliability of the multiple pumps found in other FFF systems.

 

Eclipse supports both tip injection and focus-zone injection, enabling use of a variety of FFF separation channels and even a size-exclusion chromatography column.

 

Optional features:

   •  FFF-SEC switching

   •  Dilution Control Module (DCM)

The user interface is based on a 10” multi-touch display that lets users track progress of the method, know about events or conditions that need attention, and perform any necessary manual operations such as flushing the channel. Built-in intelligence provides health indicators and guided user assistance as well as self-diagnostics procedure.

 

Front end

 

The front end for an Eclipse FFF system comprises an Agilent 1260 Infinity II pump and autosampler. These components are recognized by analytical laboratories as state-of-the-art for reliability and performance:

 

   •  Low pump ripple and pulsation

   •  No wasted sample (no need to overfill the autosampler – 100 µL are loaded for a 100 µL injection)

   •  Used in tens of thousands of laboratories around the world, including in the demanding bio/pharmaceutical industry 

 

 

Eclipse FFF channels

 

Fractionation, which is what it’s all about, takes place in the open FFF channel which incorporates a semi-permeable (ultrafiltration) membrane supported by a frit. Eclipse channels offer several key advantages including extra-rigid construction, built-in temperature regulation and built-in dilution control ports. A variety of FFF channels are available to meet different needs – see the Channels tab to learn more.

 

 

VISION

VISION software is the intelligent human interface to an FFF-MALS system built on Wyatt Technology’s Eclipse™ FFF and DAWN® MALS instruments. It comprises two primary modules:

 

   •  VISION DESIGN™ for in silico method design and optimization
   •  VISION RUN™ for running FFF methods, with continuous system monitoring and self-diagnostics to ensure no wasted runs,

      lost sample or empty solvent bottle.   

 

VISION interfaces with ASTRA software to coordinate synchronization and real-time display of light scattering data within VISION. 

 

 

Mobility EAF4

 

Mobility: measure charge and zeta potential

 

EAF4

 

Standard AF4 balances cross flow with diffusion to establish the elution velocity of particles according to size. Electrical/asymmetric-flow field-flow fractionation (EAF4) adds an electric field applied between top and bottom electrodes, and so separates by both size and particle charge (even though the sample never touches an electrode). EAF4 determines zeta potential distributions, even of multimodal and polydisperse populations.

 

 

 

 

Mobility

 

The Mobility system, which is an add-on to Eclipse, combines an innovative EAF4 channel design with outstanding software control and analysis. The Mobility module incorporates pH and conductivity sensors which are required to analyze electrophoretic mobility and related properties.

 

Use Mobility to:

 

  •  Understand if all components in the sample have similar surface chemistry

  •  Evaluate N/P ratio of drug and gene delivery nanoparticles

  •  Assess biopharmaceuticals for chemical and physical degradation 

 

View Brochure

EAF4 analysis of a mixture of polystyrene latex particles. Hydrodynamic radius us determined from 

retention time at zero applied field, while electrophoretic mobility, charge and zeta potential are determined

from the shift in retention time relative the applied field. 

 

 

Eclipse	Compatible with aqueous and organic solvents. Corrosion-resistant from pH 2 to 12.Wetted materials: 316 stainless steel, FFKM perfluoroelastomer, FR3 stainlesssteel, Vespel. Cross-flow regulation from 6.67 to 0.03 mL/min. Precision ofdetector flow rate: 0.1% (with DCM). Dedicated fraction collector output port for minimal peak broadening.
Communications	Ethernet
Inputs/Outputs	Alarm Out, Aux In with 18-bit effectiveresolution
Software	VISION softwaresuite. Comprises VISION DESIGN module for in silico method development andoptimization, and VISION RUN for running sequences and coordinating ASTRAdata acquisition.
FFF-MALS Solution	Basic FFF-MALS system includes Eclipseinstrument, one separation channel (matched to customer application), Agilent1260 Infinity II quaternary pump and autosampler, DAWN MALS instrument,VISION software suite and ASTRA light scattering analysis software.
Optional Components
Dilution Control Module	Factory-installed option. Increases sampleconcentration at the detector by up to a factor of 10 (up to 5 withnegligible loss of resolution), controlled by software.
SEC Switching Option	Factory-installedoption. Allows for automated software-controlled switching between SEC modeand FFF mode.
Mobility	Add-on option. Includes Mobility Module with pHsensor and conductivity sensor with software supported calibration, andMobility channel. Maximum operating voltage ± 30 V, maximum current 30 mA;constant current or constant voltage mode. Wetted materials: platinum,titanium, polyetherimide, alumina ceramic, PTFE, Viton, PEEK, polycarbonate.
Agilent 1260 Infinity II	Modules: VWD, MWD,DAD, FLD, fraction collector, etc.
DLS	WyattQELS™ embedded dynamic light scatteringmodule
Channels
Selection	Short, Long, Dispersion Inlet and Semi-PrepChannels available. All channels are compatible with aqueous and organicsolvents and come with DCM port. Maximum pressure 30 bar.
Temperature Regulation	Ambient to 50 °C
WettedMaterials	316 stainless steel, fused silica, FFKMperfluoroelastomer, alumina ceramic. Polycarbonate or Mylar according to theselected spacer.
Spacers	Selection rangesfrom 250 μm to 1000 μm, depending on channel. FFKM perfluoroelastomer-coatedpolycarbonate for aqueous solvents, Mylar for organic solvents.
Membranes	Pre-cut polyethersulfone or regenerated cellulosewith molecular-weight cutoff from 2 to 30 kDa. Templates available to cutother materials to size.
Dimensions	58 cm (L) x 36 cm(W) x 26 cm (H) (Eclipse instrument)

 

 

 

 

 

Accessories Guide

FFF-MALS 어플리케이션 노트