DAWN®

 

The world's Most Advanced Light Scattering Instrument For 

Absolute Characterization Of Proteins, Conjugates, Macromolecules, and Nanoparticles. 

 

   

 

The DAWN® is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and 

size of macromolecules and nanoparticles in solution. The DAWN offers the highest sensitivity, the widest range of molecular 

weight, size and concentration, and the largest selection of configurations and optional modules for enhanced capabilities. 

The DAWN is most often used in conjunction with Gel Permeation Chromatography (GPC-MALS) or Size Exclusion Chromatography 

(SEC-MALS) orField-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column 

calibration by reference standards. 

The DAWN is a accompanied by ASTRA, the most versatile software package available for analysis of online multi-angle and 

dynamic light scattering. 

1.  Dynamic Light Scattering

WyattQELS - A dynamic light scattering (DLS) detection module which integrates inside the DAWN to provide simultaneous DLS measurements in the same scattering volume. The optical fiber may be located in one of four angular positions, at 50.5°, 90°, 135° or 150°. ASTRA software automatically detects the occupied position. 

DynaPro NanoStar - Primarily used for batch DLS, the NanoStar does double duty as an on-line DLS detector by installing its optical collection fiber into any of the DAWN's four DLS positions.

 

 

2.  Temperature-Controlled Models  

    -  Ambient model

    -  Ultra-High-Temperature (UHT) model, from ambient up to +210°C

    -  Standard Peltier heated/cooled (H/C) model, temperature control from -15°C to +150°C

 

The Ultra-High-Temperature DAWN interfaces with high temperature GPC systems such as the Tosoh EcoSEC GPC-HT or the Polymer Char GPC-IR. 

 

 

3.  Laser Wavelength 

The standard 658 nm red laser may be replaced in the factory with an infrared laser operating at 785 nm. The IR laser minimizes or prevents sample fluorescence to ensure correct molar mass and size measurements on samples that fluoresce under 658 nm excitation. Please Contact Wyatt   for available laser wavelengths. Narrow bandpass fluorescence-blocking filters are also available.

 

 

4.  Optical Fibers  

Optional polarization or fluorescence-blocking filters may be installed in the DAWN. Fluorescence-blocking filters improve greatly the analysis of samples that fluoresce upon irradiation at the laser wavelength.

 

 

5.  Other Accessories 

Orbit - The Orbit helps conserve mobile phase by programmatically directing the solvent to either a waste bottle or back to the solvent reservoir. In a CG-MALS system, the Orbit is used to automatically choose wash solvents in the post-experiment cleaning cycle. 

SEC Columns - Wyatt offers silica-based columns specifically designed and tested for optimal SEC-MALS measurements of proteins and peptides. 

Solaris - Prevents growth of bacteria, fungi or algae in solvents with high efficiency and protects your system from down-time due to contamination. 

WISH - The Wyatt Injection System High-Pressure (WISH) triggers ASTRA data collection and is ideal for use in SEC-MALS without an autoinjector. Also used with an Optilab dRI detector for the determination of dn/dc values.

¹Under HPLC-SEC conditions with 7.8x300 mm columns.

²Under HPLC-SEC conditions with 7.8x300 mm Wyatt SEC protein columns.
³Under UHPLC-SEC conditions with 4.6x150 mm columns.