Product
제품
- PolyMETHYL A™
- 제품명: PolyMETHYL A™
- 용도: HIC of Proteins and Peptides
- 메이커: PolyLC
- 카달로그:
소개
PolyMETHYL A™ HPLC 컬럼
for HIC of Proteins and Peptides
HIC materials separate proteins on the basis of hydrophobic character, as does RPC. However, HIC uses totally aqueous buffers, retaining tertiary structure and biological activity. Selectivity is generally superior to that of RPC for proteins and polypeptides large enough to have significant secondary or tertiary structure. Typically, a sample is eluted with a decreasing gradient of a salt such as a sulfate or phosphate. Proteins elute in order of increasing surface hydrophobicity. Surfactants (e.g., CHAPS; octylglucoside) can be added to the mobile phase if necessary. The relative hydrophobic character of PolyPROPYL A™, PolyETHYL A™ and PolyMETHYL A™ is 100, 60 and 15, resp. HIC has greater sensitivity than other modes to the location of a modification or residue difference, especially (but not necessarily) if it involves a nonpolar group.
Use these HIC materials for:
1) Multidimensional protein purification (suggested sequence: Ion-exchange - [add salt to collected fractions] - HIC).
2) Purification of polypeptides (e.g., venoms and glycopeptides).
3) Characterization of antibodies.
4) QC analysis of proteins differing in the polarity of a single residue or modified positional variants.
Use material with 1000- or 1500-Å pores for proteins > 20 KDa. Capacity is comparable to ion-exchange. Unless a protein is known to be unusually hydrophobic, use PolyPROPYL A™
주문정보
Columns Available with 5µm Particles(Pore Size Options: -10)
| Column Dimensions | Item Number |
| 50 x 1.0mm | 051ME05-- |
| 100 x 1.0mm | 101ME05-- |
| 150 x 1.0mm | 151ME05-- |
| 35 x 2.1mm | 3.52ME05-- |
| 50 x 2.1mm | 052ME05-- |
| 100 x 2.1mm | 102ME05-- |
| 200 x 2.1mm | 202ME05-- |
| 35 x 4.6mm | 3.54ME05-- |
| 50 x 4.6mm | 054ME05-- |
| 100 x 4.6mm | 104ME05-- |
| 200 x 4.6mm | 204ME05-- |
| 100 x 9.4mm | 109ME05-- |
| 200 x 9.4mm | 209ME05-- |
| 250 x 9.4mm | 259ME05-- |
| 250 x 21.0mm | 2521ME05-- |
Columns Available with 12µm Particles(Pore Size Options: -03)
| 200 x 4.6mm | 204ME12-- |
| 200 x 9.4mm | 209ME12-- |
| 250 x 9.4mm | 259ME12-- |
| 250 x 21.0mm | 2521ME12-- |
| 250 x 50.8mm | 2550ME12-- |
Capillaries Available with 5µm Particles*(Pore Size Options: -10)**
| 50mm x 150µm | 050.15ME05-- |
| 100mm x 150µm | 100.15ME05-- |
| 150mm x 150µm | 150.15ME05-- |
| 50mm x 300µm | 050.30ME05-- |
| 100mm x 300µm | 100.30ME05-- |
| 150mm x 300µm | 150.30ME05-- |
| ** Other i.d.'s available: 75, 100, & 200 µm. Contact us for item numbers. | |
Solid Phase Extraction Cartridges - 10 packs
| SPEME1203 | 12µm, 300Å |
("J" = Javelin format; no holder needed) [NOTE: CPI-detail. To get Waters detail, add "W" to the item#]
| 10 x 1.0mm | J11GCME05-- |
| 10 x 2.0mm | J22GCME05-- |
| 10 x 3.0mm | J33GCME05-- |
| 10 x 4.0mm | JGCME05-- |
| 20 x 4.0mm | JGCME05-- -2 |
| 10 x 10mm | 110GCME05-- |
| (CH10 holder needed) | |
| 10 x 10mm | 110GCME12-- |
| (CH10 holder needed) | |
| HOLDER | CH10 |
| 30 X 21.2mm | (ask) |
| Guard Cartridges available for 21.0-mm i.d. columns. Contact Us. | |
FORMULATING A COMPLETE ITEM NUMBER
The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.
Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm,
packed with 5-µm material. If you want 1000-Å pores, then add -10 to the end. The complete item number is then 204CT0510.