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PolyETHYL A™ HPLC Column
제품명: PolyETHYL A™ HPLC Column
용도: HIC of Proteins and Peptides
메이커: PolyLC
카달로그: 다운로드


소개


PolyETHYL A™ HPLC 컬럼

 

 

for HIC of Proteins and Peptides

 

 

HIC materials separate proteins on the basis of hydrophobic character, as does RPC. However, HIC uses totally aqueous buffers, retaining tertiary structure and biological activity. Selectivity is generally superior to that of RPC for proteins and polypeptides large enough to have significant secondary or tertiary structure. Typically, a sample is eluted with a decreasing gradient of a salt such as a sulfate or phosphate. Proteins elute in order of increasing surface hydrophobicity. Surfactants (e.g., CHAPS; octylglucoside) can be added to the mobile phase if necessary. The relative hydrophobic character of PolyPROPYL A™, PolyETHYL A™ and PolyMETHYL A™ is 100, 60 and 15, resp. HIC has greater sensitivity than other modes to the location of a modification or residue difference, especially (but not necessarily) if it involves a nonpolar group. 

 


Use these HIC materials for:


   1) Multidimensional protein purification (suggested sequence: Ion-exchange - [add salt to collected fractions] - HIC).


   2) Purification of polypeptides (e.g., venoms and glycopeptides).


   3) Characterization of antibodies.


   4) QC analysis of proteins differing in the polarity of a single residue or modified positional variants.


Use material with 1000- or 1500-Å pores for proteins > 20 KDa. Capacity is comparable to ion-exchange. Unless a protein is known to be unusually hydrophobic, use PolyPROPYL A™



주문정보


Columns Available with 3µm Particles(Pore Size Options: -15)

 

 Column Dimensions  Item Number
 50 x 1.0mm  051ET03--
 100 x 1.0mm  101ET03--
 50 x 2.1mm  052ET03--
 100 x 2.1mm  102ET03--
 35 x 4.6mm  3.54ET03--
 50 x 4.6mm  054ET03--
 100 x 4.6mm  104ET03--
 100 x 9.4mm  109ET03--

 


Columns Available with 5µm Particles(Pore Size Options: -03, -10)

 

 50 x 1.0mm  051ET05--
 100 x 1.0mm  101ET05--
 150 x 1.0mm  151ET05--
 35 x 2.1mm  3.52ET05--
 50 x 2.1mm  052ET05--
 100 x 2.1mm  102ET05--
 200 x 2.1mm  202ET05--
 35 x 4.6mm  3.54ET05--
 50 x 4.6mm  054ET05--
 100 x 4.6mm  104ET05--
 200 x 4.6mm  204ET05--
 100 x 9.4mm  109ET05--
 200 x 9.4mm  209ET05--
 250 x 9.4mm  259ET05--
 250 x 21.0mm  2521ET05-- 

 


Columns Available with 12µm Particles(Pore Size Options: -03, -15) 

 

 200 x  4.6mm  204ET12--
 200 x 9.4mm  209ET12--
 250 x 9.4mm  259ET12--
 250 x 21.0mm  2521ET12--
 250 x 50.8mm  2550ET12--

 

Capillaries Available with 5µm Particles*(Pore Size Options: -03, -10)**

 

 50mm x  150µm  050.15ET05--
 100mm x 150µm  100.15ET05--
 150mm x 150µm  150.15ET05--
 50mm x 300µm  050.30ET05--
 100mm x 300µm  100.30ET05--
 150mm x 300µm  150.30ET05--
*  3-µm capillaries are available at the same price as 5-µm
  (in pore diameter of -15) Change"05--" to "03--"

 


Solid Phase Extraction Cartridges - 10 packs

 

 SPEET1203  12µm, 300Å
 SPEET1215  12µm, 1500Å

 

 

Javelin Guard Cartridges - each

("J" = Javelin format; no holder needed) [NOTE: CPI-detail. To get Waters detail,  add "W" to the item#]

 

 10 x 1.0mm  J11GCET05--
 10 x 2.0mm  J22GCET03--
 10 x 2.0mm  J22GCET05--
 10 x 3.0mm  J33GCET03--
 10 x 3.0mm  J33GCET05--
 10 x 4.0mm  JGCET03--
 10 x 4.0mm  JGCET05--
 20 x 4.0mm  JGCET03--  -2
 20 x 4.0mm  JGCET05--  -2
 10 x 10mm
  (CH10 holder needed)
 110GCET05--
 10 x  10mm
  (CH10 holder needed)
 110GCET12--
 HOLDER  CH10
 30 X 21.2mm  (ask)



FORMULATING A COMPLETE ITEM NUMBER


The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.

Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm,

packed with 5-µm material. If you want 1000-Å pores, then add -10 to the end. 

The complete item number is then 204CT0510.