PolySULFOETHYL A™

for Cation-Exchange of Peptides

 

 

This strong cation-exchange (SCX) material was developed specifically for HPLC of peptides. At pH 2.7-3.0, peptides lose their (-) charges and have net (+) charge. Thus, PolySULFOETHYL A™ is a general-purpose alternative to reversed-phase (RPC), fractionating peptides by differences in charge rather than polarity. Compared to other SCX materials based on sulfopropyl- (SP-) groups, 

PolySULFOETHYL A™ is unusually hydrophilic. This minimizes hydrophobic interactions with peptides, with high recovery and less peak tailing. Capacity is also high, permitting better retention and fractionation of the weakly basic peptides from tryptic digests. The capacity of an ion-exchange material like this is 4x that of a comparable RPC column. Therefore, ion-exchange should be the initial step of a multi-step purification.  

 

Use PolySULFOETHYL A™ for:

   1) Multidimensional HPLC of peptide mixtures, such astryptic digests in proteomics analyses (including iTRAQ® and ICAT®* reaction products).

   2) Isolation of peptides from natural products.

   3) QC and purification of synthetic peptides.

   4) Selective isolation of disulfide-linked peptides, phosphopeptides and C-terminal fragments from tryptic digests.

   5) Mapping of peptide digests (tryptic, V8, CNBr, etc.).

Proteins can be run on PolySULFOETHYL A™ columns too; at pH 3, retention is all but guaranteed. An example is the analysis of Lung Surfactant Protein on a PolySULFOETHYL A column operated in the Hydrophilic Interaction (HILIC) mode.

Standard material for peptide applications is 300-Å, in either 3- or 5-µm. The 200-Å material has about 25% greater capacity and is preferred for phosphopeptide isolation and fractionation of iTRAQ® reaction mixtures. For proteins, use 1000-Å material or the 3-µm, 1500-Å material.

Columns Available with 3µm Particles(Pore Size Options: -03, -10, -15)

 

Column Dimensions	Item Number
50 x 1.0mm	051SE03--
100 x 1.0mm	101SE03--
50 x 2.1mm	052SE03--
100 x 2.1mm	102SE03--
35 x 4.6mm	3.54SE03--
50 x 4.6mm	054SE03--
100 x 4.6mm	104SE03--
100 x 9.4mm	109SE03--

 

Columns Available with 5µm Particles(Pore Size Options: -02, -03, -10)

 

Column Dimensions	Item Number
50 x 1.0mm	051SE05--
100 x 1.0mm	101SE05--
150 x 1.0mm	151SE05--
35 x 2.1mm	3.52SE05--
50 x 2.1mm	052SE05--
100 x 2.1mm	102SE05--
200 x 2.1mm	202SE05--
35 x 4.6mm	3.54SE05--
50 x 4.6mm	054SE05--
100 x 4.6mm	104SE05--
200 x 4.6mm	204SE05--
100 x 9.4mm	109SE05--
200 x 9.4mm	209SE05--
250 x 9.4mm	259SE05--
250 x 21.0mm	2521SE05--

   

Columns Available with 12µm Particles(Pore Size Options: -03, -15) 

 

Column Dimensions	Item Number
200 x 4.6mm	204SE12--
200 x 9.4mm	209SE12--
250 x 9.4mm	259SE12--
250 x 21.0mm	2521SE12--
250 x 50.8mm	2550SE12--

 

Capillaries Available with 5µm Particles*(Pore Size Options: -02, -03, -10)**

50mm x  150µm	050.15SE05--
100mm x 150µm	100.15SE05--
150mm x 150µm	150.15SE05--
50mm x 300µm	050.30SE05--
100mm x 300µm	100.30SE05--
150mm x 300µm	150.30SE05--

 

Solid Phase Extraction Cartridges - 10 packs

SPESE1203	12µm, 300Å
SPESE1215	12µm, 1500Å
SPESE2003	20µm, 300Å
SPESE2015	20µm, 1500Å

 

 

Javelin Guard Cartridges - each

("J" = Javelin format; no holder needed) [NOTE: CPI-detail. To get Waters detail,  add "W" to the item#]

 

10 x 1.0mm	J11GCSE05--
10 x 2.0mm	J22GCSE03--
10 x 2.0mm	J22GCSE05--
10 x 3.0mm	J33GCSE03--
10 x 3.0mm	J33GCSE05--
10 x 4.0mm	JGCSE03--
10 x 4.0mm	JGCSE05--
20 x 4.0mm	JGCSE03--  -2
20 x 4.0mm	JGCSE05--  -2
10 x 10mm   (CH10 holder needed)	110GCSE05--
10 x  10mm   (CH10 holder needed)	100GCSE12--
HOLDER	CH10
20 X 10.0mm	(ask)

FORMULATING A COMPLETE ITEM NUMBER

The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.

Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm,

packed with 5-µm material. If you want 1000-Å pores, then add -10 to the end. 

The complete item number is then 204CT0510.