Product
소개
PolyWAX LP™ HPLC Column
for Anion-Exchange of Proteins and Nucleic Acids
1. Isocratic separation of amino acids, peptides, and proteins.
2. Selective isolation and separation of phosphopeptides.
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Most proteins have isoelectric points below 7, and are best purified or analyzed by anion-exchange chromatography. PolyWAX LP™ is a hydrophilic weak anion-exchange (WAX) material developed by PolyLC for HPLC of enzymes and other proteins. Selectivity is excellent, with high or quantitative recovery of applied biological activity. Most anion-exchange materials based on polyethyleneimine (PEI) are prepared with the conventional branched polymer.
PolyWAX LP™ is prepared with linear PEI, which confers greater selectivity and recovery.
The following example demonstrates the ability of PolyWAX LP™ to separate proteins differing by a single phosphate group.
Anion-exchange is the method of choice for resolution of oligonucleotides and their analogs > 15 bases. It is also much faster and convenient than PAGE gels for purification of the double-stranded DNA products from PCR reactions.
Use PolyWAX LP™ for:
1) Purification of acidic proteins and polypeptides from natural products.
2) Analysis and purification of oligonucleotides and their analogs as well as amplified PCR products.
3) In proteomics, predigest fractionation of intact proteins via mixed-bed ion-exchange.
4) Anion-exchange of small, acidic solutes.
For small solutes, use our 100-Å material. For peptides, use 300 Å. For proteins > 20 KDa, we recommend pore diameters of at least 1000 Å for optimal selectivity and efficiency. Our 3-µm, 1500-Å material affords superior separations of closely-related protein variants.
주문정보
Columns Available with 3µm Particles(Pore Size Options: -01, -03, -10, -15)
Column Dimensions | Item Number |
50 x 1.0mm | 051WX03-- |
100 x 1.0mm | 101WX03-- |
50 x 2.1mm | 052WX03-- |
100 x 2.1mm | 102WX03-- |
35 x 4.6mm | 3.54WX03-- |
50 x 4.6mm | 054WX03-- |
100 x 4.6mm | 104WX03-- |
100 x 9.4mm | 109WX03-- |
Columns Available with 5µm Particles(Pore Size Options: -01, -03, -10)
Column Dimensions | Item Number |
50 x 1.0mm | 051WX05-- |
100 x 1.0mm | 101WX05-- |
150 x 1.0mm | 151WX05-- |
35 x 2.1mm | 3.52WX05-- |
50 x 2.1mm | 052WX05-- |
100 x 2.1mm | 102WX05-- |
200 x 2.1mm | 202WX05-- |
35 x 4.6mm | 3.54WX05-- |
50 x 4.6mm | 054WX05-- |
100 x 4.6mm | 104WX05-- |
200 x 4.6mm | 204WX05-- |
100 x 9.4mm | 109WX05-- |
200 x 9.4mm | 209WX05-- |
250 x 9.4mm | 259WX05-- |
250 x 21.0mm | 2521WX05-- |
Columns Available with 12µm Particles(Pore Size Options: -01, -03, -15)
Column Dimensions | Item Number |
200 x 4.6mm | 204WX12-- |
200 x 9.4mm | 209WX12-- |
250 x 9.4mm | 259WX12-- |
250 x 21.0mm | 2521WX12-- |
250 x 50.8mm | 2550WX12-- |
Capillaries Available with 5µm Particles*(Pore Size Options: -01, -03, -10)**
50mm x 150µm | 050.15WX05-- |
100mm x 150µm | 100.15WX05-- |
150mm x 150µm | 150.15WX05-- |
50mm x 300µm | 050.30WX05-- |
100mm x 300µm | 100.30WX05-- |
150mm x 300µm | 150.30WX05-- |
Solid Phase Extraction Cartridges - 10 packs
SPEWX1201 | 12µm, 100Å |
SPEWX1203 | 12µm, 300Å |
SPEWX1215 | 12µm, 1500Å |
SPEWX2003 | 20µm, 300Å |
SPEWX2015 | 20µm, 1500Å |
("J" = Javelin format; no holder needed) [NOTE: CPI-detail. To get Waters detail, add "W" to the item#]
10 x 1.0mm | J11GCWX05-- |
10 x 2.0mm | J22GCWX03-- |
10 x 2.0mm | J22GCWX05-- |
10 x 3.0mm | J33GCWX03-- |
10 x 3.0mm | J33GCWX05-- |
10 x 4.0mm | JGCWX03-- |
10 x 4.0mm | JGCWX05-- |
20 x 4.0mm | JGCWX03-- -2 |
20 x 4.0mm | JGCWX05-- -2 |
10 x 10mm (CH10 holder needed) | 110GCWX05-- |
10 x 10mm (CH10 holder needed) | 110GCWX12-- |
HOLDER | CH10 |
30 X 21.2mm | (ask) |
FORMULATING A COMPLETE ITEM NUMBER
The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.
Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm,
packed with 5-µm material. If you want 1000-Å pores, then add -10 to the end.
The complete item number is then 204CT0510