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PolyWAX LP™ HPLC Column
제품명: PolyWAX LP™ HPLC Column
용도: Anion-Exchange of Proteins and Nucleic Acids
메이커: PolyLC
카달로그: 다운로드


소개


PolyWAX LP™ HPLC Column

 


for Anion-Exchange of Proteins and Nucleic Acids

 

 


1. Isocratic separation of amino acids, peptides, and proteins.


2. Selective isolation and separation of phosphopeptides.
....Click Here for details!

 


Most proteins have isoelectric points below 7, and are best purified or analyzed by anion-exchange chromatography. PolyWAX LP™ is a hydrophilic weak anion-exchange (WAX) material developed by PolyLC for HPLC of enzymes and other proteins. Selectivity is excellent, with high or quantitative recovery of applied biological activity. Most anion-exchange materials based on polyethyleneimine (PEI) are prepared with the conventional branched polymer. 


PolyWAX LP™ is prepared with linear PEI, which confers greater selectivity and recovery.  


The following example demonstrates the ability of PolyWAX LP™ to separate proteins differing by a single phosphate group.

 


 


 



Anion-exchange is the method of choice for resolution of oligonucleotides and their analogs > 15 bases. It is also much faster and convenient than PAGE gels for purification of the double-stranded DNA products from PCR reactions.



Use PolyWAX LP™ for:


   1) Purification of acidic proteins and polypeptides from natural products.


   2) Analysis and purification of oligonucleotides and their analogs as well as amplified PCR products.


   3) In proteomics, predigest fractionation of intact proteins via mixed-bed ion-exchange.


   4) Anion-exchange of small, acidic solutes.



For small solutes, use our 100-Å material. For peptides, use 300 Å. For proteins > 20 KDa, we recommend pore diameters of at least 1000 Å for optimal selectivity and efficiency. Our 3-µm, 1500-Å material affords superior separations of closely-related protein variants.


주문정보

Columns Available with 3µm Particles(Pore Size Options: -01, -03, -10, -15)

 

 Column Dimensions  Item Number
 50 x 1.0mm  051WX03--
 100 x 1.0mm  101WX03--
 50 x 2.1mm  052WX03--
 100 x 2.1mm  102WX03--
 35 x 4.6mm  3.54WX03--
 50 x 4.6mm  054WX03--
 100 x 4.6mm  104WX03--
 100 x 9.4mm  109WX03--

 


Columns Available with 5µm Particles(Pore Size Options: -01, -03, -10)

 

 Column Dimensions  Item Number
 50 x 1.0mm  051WX05--
 100 x 1.0mm  101WX05--
 150 x 1.0mm  151WX05--
 35 x 2.1mm  3.52WX05--
 50 x 2.1mm  052WX05--
 100 x 2.1mm  102WX05--
 200 x 2.1mm  202WX05--
 35 x 4.6mm  3.54WX05--
 50 x 4.6mm  054WX05--
 100 x 4.6mm  104WX05--
 200 x 4.6mm  204WX05--
 100 x 9.4mm  109WX05--
 200 x 9.4mm  209WX05--
 250 x 9.4mm  259WX05--
 250 x 21.0mm  2521WX05--

 


Columns Available with 12µm Particles(Pore Size Options: -01, -03, -15) 

 

 Column Dimensions  Item Number
 200 x 4.6mm  204WX12--
 200 x 9.4mm  209WX12--
 250 x 9.4mm  259WX12--
 250 x 21.0mm  2521WX12--
 250 x 50.8mm  2550WX12--

 


Capillaries Available with 5µm Particles*(Pore Size Options: -01, -03, -10)**

 

 50mm x  150µm  050.15WX05--
 100mm x 150µm  100.15WX05--
 150mm x 150µm  150.15WX05--
 50mm x 300µm  050.30WX05--
 100mm x 300µm  100.30WX05--
 150mm x 300µm  150.30WX05--

 


Solid Phase Extraction Cartridges - 10 packs

 

 SPEWX1201  12µm, 100Å
 SPEWX1203  12µm,  300Å
 SPEWX1215  12µm,  1500Å
 SPEWX2003  20µm,  300Å
 SPEWX2015  20µm,  1500Å

 


Javelin Guard Cartridges - each

("J" = Javelin format; no holder needed) [NOTE: CPI-detail. To get Waters detail, add "W" to the item#] 

 

 10 x 1.0mm  J11GCWX05--
 10 x 2.0mm  J22GCWX03--
 10 x 2.0mm  J22GCWX05--
 10 x 3.0mm  J33GCWX03--
 10 x 3.0mm  J33GCWX05--
 10 x 4.0mm  JGCWX03--
 10 x 4.0mm  JGCWX05--
 20 x 4.0mm  JGCWX03--  -2
 20 x 4.0mm  JGCWX05--  -2
 10 x 10mm (CH10 holder   needed)  110GCWX05--
 10 x  10mm (CH10 holder needed)  110GCWX12--
 HOLDER  CH10
 30 X 21.2mm  (ask)


 


FORMULATING A COMPLETE ITEM NUMBER


The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.

Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm,

packed with 5-µm material. If you want 1000-Å pores, then add -10 to the end. 

The complete item number is then 204CT0510