PolyGLYCOPLEX A™

 

for HILIC of Complex Carbohydrates

 

  

This neutral material has the unusually high capacity needed to retain complex carbohydrates in the HILIC mode. 

Columns can frequently be operated with just acetonitrile and water, although charged carbohydrates may require inclusion of some salt such as ammonium acetate. These conditions are convenient for isolation of carbohydrates or direct flow to a mass spectrometer. 

Selectivity is good for both native glycans and derivatives such as those with the 2-aminopyridine (PA-) fluorophore. 

Oligosaccharide mixtures can often be resolved isocratically, although gradients are recommended for especially varied samples. 

Sialylated and asialo- glycans can be resolved using the same running conditions.  

In some cases selectivity compares favorably with that of HPAEC with PAD detection, and both running conditions and equipment maintenance are far more convenient. 

 

 

 

 

 

 

 

Columns with 3µm Particles

 

Column Dimensions	Item Number
50 x 1.0mm	051GL0300
100 x 1.0mm	101GL0300
50 x 2.1mm	052GL0300
100 x 2.1mm	102GL0300
35 x 4.6mm	3.54GL0300
50 x 4.6mm	054GL0300
100 x 4.6mm	104GL0300
100 x 9.4mm	109GL0300

 

Columns with 5µm Particles

 

Column Dimensions	Item Number
50 x 1.0mm	051GL0500
100 x 1.0mm	101GL0500
150 x 1.0mm	151GL0500
35 x 2.1mm	3.52GL0500
50 x 2.1mm	052GL0500
100 x 2.1mm	102GL0500
200 x 2.1mm	202GL0500
35 x 4.6mm	3.54GL0500
50 x 4.6mm	054GL0500
100 x 4.6mm	104GL0500
200 x 4.6mm	204GL0500
100 x 9.4mm	109GL0500
200 x 9.4mm	209GL0500
250 x 9.4mm	259GL0500
250 x 21.0mm	2521GL0500

 

Columns Available with 12µm Particles

 

Column Dimensions	Item Number
200 x 4.6mm	204GL1200
200 x 9.4mm	209GL1200
250 x 9.4mm	259GL1200
250 x 21.0mm	2521GL1200
250 x 50.8mm	2550GL1200

 

Capillaries Available with 5µm Particles*(Pore Size Options: -02, -03, -10)**

 

50mm x  150µm	050.15GL0500
100mm x 150µm	100.15GL0500
150mm x 150µm	150.15GL0500
50mm x 300µm	050.30GL0500
100mm x 300µm	100.30GL0500
150mm x 300µm	150.30GL0500
*  3-µm capillaries are available at the same price as 5-µm
Change"0500"  to "0300"

 

 

Solid Phase Extraction Cartridges - 10 packs

 

SPEGL1201	12µm
SPEGL2001	20µm,  100Å

 

Javelin Guard Cartridges - each

("J" = Javelin format; no holder needed) [NOTE: CPI-detail. To get Waters detail, add "W" to the item#]

 

10 x 1.0mm	J11GCGL0500
10 x 2.0mm	J22GCGL0300
10 x 2.0mm	J22GCGL0500
10 x 3.0mm	J33GCGL0300
10 x 3.0mm	J33GCGL0500
10 x 4.0mm	JGCGL0300
10 x 4.0mm	JGCGL0500
20 x 4.0mm	JGCGL0300  -2
20 x 4.0mm	JGCGL0500  -2
10 x 10mm	110GCGL0500
(CH10  holder needed)
10 x 10mm	100GCGL12--
(CH10  holder needed)
HOLDER	CH10
20 X 10.0mm	(ask)

 

 

FORMULATING A COMPLETE ITEM NUMBER

The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.

Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm,
packed with 5-µm material. If you want 1000-Å pores, then add -10 to the end. 

The complete item number is then 204CT0510.