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PolyHYDROXYETHYL A™ HPLC Column
제품명: PolyHYDROXYETHYL A™ HPLC Column
용도: Hydrophilic Interaction & Size Exclusion
메이커: PolyLC
카달로그: 다운로드


소개

 

PolyHYDROXYETHYL A™ HPLC 컬럼

 


for Hydrophilic Interaction and Size Exclusion HPLC 

 

 


This neutral, polar material was developed specifically for Hydrophilic Interaction Chromatography (HILIC).  


HILIC is a variant of normal phase chromatography that is performed with a polar stationary phase and a partly aqueous mobile phase.  


This permits normal-phase separations of peptides, nucleic acids, carbohydrates, some proteins, and polar solutes in general. In comparison with other materials used for HILIC, PolyHYDROXYETHYL A™ affords sharper peaks and better selectivity and recovery. Its great polarity means less organic solvent is needed to get retention. 

 


Use PolyHYDROXYETHYL A™ for: 


  1) Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma 

    or crude extracts.


  2) Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.).


  3) Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests.


  4) HPLC of solutes that aren't soluble in aqueous media (membrane proteins; phospholipids).


  5) Eliminating detergents, lipids, and salts from a sample.


  6) Oligonucleotides and their analogs.

 


In the absence of organic solvent, PolyHYDROXYETHYL A™ functions in the Size Exclusion Chromatography (SEC) mode.

For proteins and peptides, use 200- or 300-Å material. For polar small solutes, try our premium 3-µm, 100-Å material.


주문정보

Columns with 3µm Particles (Pore Size Options: -006, -01,-03, -05, -10, -15)

  

 

 Column Dimensions  Item Number
 50 x 1.0mm  051HY03--
 100 x 1.0mm  101HY03--
 50 x 2.1mm  052HY03--
 100 x 2.1mm  102HY03--
 35 x 4.6mm  3.54HY03--
 50 x 4.6mm  054HY03--
 100 x 4.6mm  104HY03--
 100 x 9.4mm  109HY03--

 


Columns with 5µm Particles (Pore Size Options: -006, -01, -02, -03, -05, -10)

  

 

 Column Dimensions  Item Number
 50 x 1.0mm  051HY05--
 100 x 1.0mm  101HY05--
 150 x 1.0mm  151HY05--
 35 x 2.1mm  3.52HY05--
 50 x 2.1mm  052HY05--
 100 x 2.1mm  102HY05--
 200 x 2.1mm  202HY05--
 35 x 4.6mm  3.54HY05--
 50 x 4.6mm  054HY05--
 100 x 4.6mm  104HY05--
 200 x 4.6mm  204HY05--
 100 x 9.4mm  109HY05--
 200 x 9.4mm  209HY05--
 250 x 9.4mm  259HY05--
 250 x 21.0mm  2521HY05--

 


Columns Available with 12µm Particles (Pore Size Options: -006, -01, -03, -15) 

  

 

 Column Dimensions  Item Number
 200 x 4.6mm  204HY12--
 200 x 9.4mm  209HY12--
 250 x 9.4mm  259HY12--
 250 x 21.0mm  2521HY12--
 250 x 50.8mm  2550HY12--

 


Capillaries Available with 5µm Particles* (Pore Size Options: -02, -03, -10)**

  

 

 50mm x  150µm  050.15HY05--
 100mm x 150µm  100.15HY05--
 150mm x 150µm  150.15HY05--
 50mm x 300µm  050.30HY05--
 100mm x 300µm  100.30HY05--
 150mm x 300µm  150.30HY05--

 


Solid Phase Extraction Cartridges - 10 packs

  

 

 SPEHY1201  12µm, 100Å
 SPEHY1203  12µm,  300Å
 SPEHY1215  12µm,  1500Å
 SPEHY2001  20µm,  100Å
 SPEHY2003  20µm,  300Å

 


Javelin Guard Cartridges - each

("J" = Javelin format; no holder needed) [NOTE: CPI-detail. To get Waters detail, add "W" to the item#]

   

 

 10 x 1.0mm  J11GCHY05--
 10 x 2.0mm  J22GCHY03--
 10 x 2.0mm  J22GCHY05--
 10 x 3.0mm  J33GCHY03--
 10 x 3.0mm  J33GCHY05--
 10 x 4.0mm  JGCHY03--
 10 x 4.0mm  JGCHY05--
 20 x 4.0mm  JGCHY03--  -2
 20 x 4.0mm  JGCHY05--  -2
 10 x 10mm  110GCHY05--
 (CH10  holder needed)
 10 x 10mm  100GCHY12--
 (CH10  holder needed)
 HOLDER  CH10
 20 X 10.0mm  (ask)


 

 

FORMULATING A COMPLETE ITEM NUMBER


The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.

Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm,
packed with 5-µm material. If you want 1000-Å pores, then add -10 to the end. The complete
item number is then 204CT0510.