Product
소개
PolyHYDROXYETHYL A™ HPLC 컬럼
for Hydrophilic Interaction and Size Exclusion HPLC
This neutral, polar material was developed specifically for Hydrophilic Interaction Chromatography (HILIC).
HILIC is a variant of normal phase chromatography that is performed with a polar stationary phase and a partly aqueous mobile phase.
This permits normal-phase separations of peptides, nucleic acids, carbohydrates, some proteins, and polar solutes in general. In comparison with other materials used for HILIC, PolyHYDROXYETHYL A™ affords sharper peaks and better selectivity and recovery. Its great polarity means less organic solvent is needed to get retention.
Use PolyHYDROXYETHYL A™ for:
1) Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma
or crude extracts.
2) Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.).
3) Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests.
4) HPLC of solutes that aren't soluble in aqueous media (membrane proteins; phospholipids).
5) Eliminating detergents, lipids, and salts from a sample.
6) Oligonucleotides and their analogs.
In the absence of organic solvent, PolyHYDROXYETHYL A™ functions in the Size Exclusion Chromatography (SEC) mode.
For proteins and peptides, use 200- or 300-Å material. For polar small solutes, try our premium 3-µm, 100-Å material.
주문정보
Columns with 3µm Particles (Pore Size Options: -006, -01,-03, -05, -10, -15)
Column Dimensions | Item Number |
50 x 1.0mm | 051HY03-- |
100 x 1.0mm | 101HY03-- |
50 x 2.1mm | 052HY03-- |
100 x 2.1mm | 102HY03-- |
35 x 4.6mm | 3.54HY03-- |
50 x 4.6mm | 054HY03-- |
100 x 4.6mm | 104HY03-- |
100 x 9.4mm | 109HY03-- |
Columns with 5µm Particles (Pore Size Options: -006, -01, -02, -03, -05, -10)
Column Dimensions | Item Number |
50 x 1.0mm | 051HY05-- |
100 x 1.0mm | 101HY05-- |
150 x 1.0mm | 151HY05-- |
35 x 2.1mm | 3.52HY05-- |
50 x 2.1mm | 052HY05-- |
100 x 2.1mm | 102HY05-- |
200 x 2.1mm | 202HY05-- |
35 x 4.6mm | 3.54HY05-- |
50 x 4.6mm | 054HY05-- |
100 x 4.6mm | 104HY05-- |
200 x 4.6mm | 204HY05-- |
100 x 9.4mm | 109HY05-- |
200 x 9.4mm | 209HY05-- |
250 x 9.4mm | 259HY05-- |
250 x 21.0mm | 2521HY05-- |
Columns Available with 12µm Particles (Pore Size Options: -006, -01, -03, -15)
Column Dimensions | Item Number |
200 x 4.6mm | 204HY12-- |
200 x 9.4mm | 209HY12-- |
250 x 9.4mm | 259HY12-- |
250 x 21.0mm | 2521HY12-- |
250 x 50.8mm | 2550HY12-- |
Capillaries Available with 5µm Particles* (Pore Size Options: -02, -03, -10)**
50mm x 150µm | 050.15HY05-- |
100mm x 150µm | 100.15HY05-- |
150mm x 150µm | 150.15HY05-- |
50mm x 300µm | 050.30HY05-- |
100mm x 300µm | 100.30HY05-- |
150mm x 300µm | 150.30HY05-- |
Solid Phase Extraction Cartridges - 10 packs
SPEHY1201 | 12µm, 100Å |
SPEHY1203 | 12µm, 300Å |
SPEHY1215 | 12µm, 1500Å |
SPEHY2001 | 20µm, 100Å |
SPEHY2003 | 20µm, 300Å |
("J" = Javelin format; no holder needed) [NOTE: CPI-detail. To get Waters detail, add "W" to the item#]
10 x 1.0mm | J11GCHY05-- |
10 x 2.0mm | J22GCHY03-- |
10 x 2.0mm | J22GCHY05-- |
10 x 3.0mm | J33GCHY03-- |
10 x 3.0mm | J33GCHY05-- |
10 x 4.0mm | JGCHY03-- |
10 x 4.0mm | JGCHY05-- |
20 x 4.0mm | JGCHY03-- -2 |
20 x 4.0mm | JGCHY05-- -2 |
10 x 10mm | 110GCHY05-- |
(CH10 holder needed) | |
10 x 10mm | 100GCHY12-- |
(CH10 holder needed) | |
HOLDER | CH10 |
20 X 10.0mm | (ask) |
FORMULATING A COMPLETE ITEM NUMBER
The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.
Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm,
packed with 5-µm material. If you want 1000-Å pores, then add -10 to the end. The complete
item number is then 204CT0510.