Product
제품
- PolyCAT A™ HPLC Column
- 제품명: PolyCAT A™ HPLC Column
- 용도: WCX of Proteins
- 메이커: PolyLC
- 카달로그:
소개
PolyCAT A™ HPLC 컬럼
for Cation-Exchange of Proteins
PolyCAT A™ is made through a unique process for attaching poly(aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Use PolyCAT A™ for:
1) Protein variants involving deamidation, PEGylation, position of attachment, desialylation, etc. It is widely used by the biotechnology industry.
2) Hemoglobin variant analysis by clinical chemistry labs.
3) Proteins with pI above 6.0 (5.0 in special cases).
5) Histones.
Since PolyCAT A™ is a weak cation-exchange (WCX) material, it is used at pH values above 4. A gradient to unbuffered acetic acid will uncharge PolyCAT A™, permitting the elution of proteins in a volatile solvent. See our posteron this subject.
Peptides can be run on PolyCAT A™ if they contain at least two excess positive charges above pH 4. More weakly basic peptides, such as tryptic fragments, are not reliably retained and should be run on PolySULFOETHYL A™ at pH = 2.7 - 3.0
For proteins larger than 20 KDa, we recommend the use of pore diameters of at least 1000 Å for optimal selectivity and efficiency. Our 3-µm material with 1000- or 1500-Å pores is the finest cation-exchanger available for protein separations.
주문정보
| Column Dimensions | Item Number |
| 50 x 1.0mm | 051CT03-- |
| 100 x 1.0mm | 101CT03-- |
| 50 x 2.1mm | 052CT03-- |
| 100 x 2.1mm | 102CT03-- |
| 35 x 4.6mm | 3.54CT03-- |
| 50 x 4.6mm | 054CT03-- |
| 100 x 4.6mm | 104CT03-- |
| 100 x 9.4mm | 109CT03-- |
Columns Available with 5µm Particles (Pore Size Options: -03, -10)
| Column Dimensions | Item Number |
| 50 x 1.0mm | 051CT05-- |
| 100 x 1.0mm | 101CT05-- |
| 150 x 1.0mm | 151CT05-- |
| 35 x 2.1mm | 3.52CT05-- |
| 50 x 2.1mm | 052CT05-- |
| 100 x 2.1mm | 102CT05-- |
| 200 x 2.1mm | 202CT05-- |
| 35 x 4.6mm | 3.54CT05-- |
| 50 x 4.6mm | 054CT05-- |
| 100 x 4.6mm | 104CT05-- |
| 200 x 4.6mm | 204CT05-- |
| 100 x 9.4mm | 109CT05-- |
| 200 x 9.4mm | 209CT05-- |
| 250 x 9.4mm | 259CT05-- |
| 250 x 21.0mm | 2521CT05-- |
Columns Available with 12µm Particles (Pore Size Options: -03, -15)
| Column Dimensions | Item Number |
| 200 x 4.6mm | 204CT12-- |
| 200 x 9.4mm | 209CT12-- |
| 250 x 9.4mm | 259CT12-- |
| 250 x 21.0mm | 2521CT12-- |
| 250 x 50.8mm | 2550CT12-- |
Capillaries Available with 5µm Particles* (Pore Size Options: -03, -10)**
| 50mm x 150µm | 050.15CT05-- |
| 100mm x 150µm | 100.15CT05-- |
| 150mm x 150µm | 150.15CT05-- |
| 50mm x 300µm | 050.30CT05-- |
| 100mm x 300µm | 100.30CT05-- |
| 150mm x 300µm | 150.30CT05-- |
Solid Phase Extraction Cartridges - 10 packs
| SPECT1203 | 12µm, 300Å |
| SPECT1215 | 12µm, 1500Å |
| SPECT2003 | 20µm, 300Å |
| SPECT2015 | 20µm, 1500Å |
FORMULATING A COMPLETE ITEM NUMBER
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.
Example of a complete item number: 204CT05-- designates a PolyCAT A column, 200x4.6mm, packed with 5-µm material.
If you want 1000-Å pores, then add -10 to the end. The complete item number is then 204CT0510.