Recombinant 2019-nCoV Antigens

2019-nCoV is a new type of bat coronavirus identified as the cause of an outbreak of respiratory illness first detected in Wuhan,

China. Early on, many of the patients in the outbreak in Wuhan, China reportedly had some link to a large seafood and animal

market, suggesting animal-to-person spread. However, a growing number of patients reportedly have not had exposure to 

animal markets, indicating person-to-person spread is occurring. At this time, it’s unclear how easily or sustainably this virus is 

spreading between people.

The coronavirinae family consists of four genera based on their genetic properties, including genus Alphacoronavirus, genus 

Betacoronavirus, genus Gammacoronavirus, and genus Deltacoronavirus. Host range and tissue tropism show a lot of variation

among different CoVs. Generally, the Alphacoronavirus and Betacoronavirus can infect mammals, and the Gammacoronavirus 

and Deltacoronavirus can infect birds, but some of them can also infect mammals. 2019-nCoV, SARS coronavirus and MERS 

coronavirus all belong to the Betacoronavirus genus and are zoonotic pathogens that can cause severe respiratory diseases in 

humans.

Genome analysis of 2019-nCoV showed that 2019-nCoV was slightly different from SARS CoV and MERS CoV, but the 

functionally important ORFs, ORF1a and ORF1b, and major structural proteins such as the spike (S), membrane (M), envelop (E) 

and nucleic capsid (N) proteins are well annotated. Four structural proteins are essential for virion assembly and infection of 

CoVs. Homotrimers of S proteins make up the spike on the surface of virus particles and it is the sole viral membrane protein 

responsible for cell entry. It binds to the receptor on the target cell and mediates subsequent virus-cell fusion. It is also the key

target for vaccine design. The M protein has three transmembrane domains and shapes the virions, promotes membrane 

curvature, and binds to the nucleocapsid. The E protein plays a role invirus assembly and release, and it is required for 

pathogenesis. The N protein contains two domains, both of them can bind virus RNA genome via different mechanisms. It is 

reported that N protein is an antagonist of interferon and viral encoded repressor (VSR) of RNA interference (RNAi), which 

benefit the viral replication. 

2019-nCoV spike protein, which belongs to class I virus fusion protein, contains two subunits, S1 and S2. S1 mainly contains 

receptor binding domain (RBD), which is responsible for identifying cell receptors. S2 contains the basic elements needed for

the membrane fusion process. It has been reported that 2019-ncov spike protein interacts with human ACE2 to infect human 

respiratory epithelial cells.

Creative Diagnostics now can provide recombinant 2019-nCoV spike, nucleocapsid antigens. These antigens can be used for

scientific research, detection of novel coronavirus and preparation of neutralizing antibodies. All of our antigens are produced

using a standardized production process to ensure the highest quality and are performance guaranteed for the applications 

listed on the detailed datasheets.  

Recombinant 2019-nCoV Antigens Product List