The role of electrostatics in protein-protein interactions of a monoclonal antibody

Antibody formulation stability is strongly affected by intermolecular

interactions. In this article, researchers used static, dynamic, and

electrophoretic light scattering to assess molecular contributions to 

protein-protein interactions, including excluded volume repulsion, 

electrostatic attraction, and other short and long range forces. The Calypso® 

and miniDAWN® enabled them to measure the second virial coefficient 

(A2 or B22) as a function of pH and ionic strength. The diffusion interaction 

parameter (kD) was measured under the same conditions via dynamic light 

scattering using the DynaPro® Plate Reader. These values were then

correlatedto the electrophoretic mobility measured by electrophoretic light 

scattering in the Mobius®.

Roberts, D.; Keeling, R.; Tracka, M.; van der Walle, C.F.; Uddin, S.; Warwicker, J.; Curtis, 

R. Molecular Pharmaceutics  2014, 11, 2475–2489. doi:10.1021/mp5002334