the biological and biopharmaceutical sciences

This article provides a brief introduction to the four essential forms of light

scattering utilized in the life sciences: on-line MALS, CG-MALS, DLS, and 

ELS, used to determine molar mass, size, native oligomeric and conjugation 

state; characterize interactions, aggregation and stability; and determine net 

charge or zeta potential. It then proceeds to provide a variety of examples 

of how each technique is used. The examples are drawn from recent 

literature, and in addition several tables provide brief descriptions of the key 

results obtained by means of light scattering over a more extensive set of 

recent publications. The author offers some helpful critiques and guidelines 

for LS users.

Minton, A.P. Analytical Biochemistry  2016, 501, 4-22. doi:10.1016/j.ab.2016.02.007