[Ribotyping] 미생물 동정 및 특성화
|Date : 2019.12.19|
|분류 : 동정및특성화|
RiboPrinter® System Automated Molecular Method For Powerful Microbial Monitoring
" More than 16S Sequencing "
The RiboPrinter® System automates restriction fragment lengthpolymorphism (RFLP) analysis and targets the RNA-coding region
of the bacterial genome. Restriction enzymes, such as EcoRI orPvuII, cut bacterial DNA into fragments that are processed to form
a characteristic banding pattern or “fingerprint.” The system captures an image of the banding pattern and digitizes it as a
RiboPrint™pattern. This pattern can then be compared to a reference databaseof patterns from historic samples previously run at
a particular location or on a specific instrument.
Rather than just analyzing the region that encodes the 16S rRNAsequence, the RiboPrinter® System investigates the regions
encoding the 5S, 16S and 23S sequences, as well as the spacerregions and flanking genes on either side. This rich depth of
information is what allows highly precise differentiation among strainsof the same species, even those with the same 16S
Fragments from each sample form abanding pattern according to size.
Banding patterns from each sample,along with marker lanes, are capturedin a digitized gel image.
The final report displays the RiboPrint™ pattern along with associated data for each sample.
" Distinguish More than 290 Genera and Over 1,700 Species "
RiboPrint™ patterns characterize environmentalisolates, pathogens, spoilage organisms, controlstrains, beneficial organisms or any
bacteria that are important to quality control professionalsin the pharmaceutical and food industries,or to epidemiologists and
other public healthprofessionals.
Beyond reliable taxonomic identification, the RiboPrinter® Systemdistinguishes individual strains,
providing the granularity of information needed to track, trend and control your environment.
How it Works
Load up to 8 samples at a time in the automated unit.
Genetic fingerprints (RiboPrint™patterns) are generated for each sample in 8 hours.